地塞米松治疗自身免疫性感音神经性耳聋的实验研究

THE EXPENMENTAL STUDY OF THE EFFECT OF DEXAMETHASON ON EXPERIMENTAL AUTOIMMUNE SENSORINEURAL NEARING LOSS

本实验应用豚鼠内耳母抗原液免疫豚鼠产生自身免疫性感音神经性耳聋(AISNHL),后用地塞米松治疗1周,并行听觉脑干诱发电位阈(ABRT)测定和内耳形态学观察。我们发现免疫后豚鼠ABRT升高到60.8±531dBSPL、耳蜗第3回外毛细胞损失达111.0±55.8个;而应用地塞米松治疗后的豚鼠ABRT则下降为48.8±6.69dBSPL。耳蜗第3回外毛细胞损失仅为57.25±18个,证实地塞米松对AISNHL有治疗效果,它可使内耳组织恢复,ABRT明显改善,其机制尚不明了。

In this experiment, Animal model of guinea pig's experimental Autoimmune sensorineural hearing Loss was established by injecting crud inner ear antigen (CIEAg), Dexamethason was used to tret the deaf guinea pigs for one week, the ABRT(Acoustic Brain—stem Reaction Threshold) of guinea pigs was measured and the morphological change of the guinea pigs inner ear, tissue was observd. We found that the deaf guinea pig's ABRT rose distinctly to 60. 8±5.31 dBspl and inner ear hair cell showed severe damage. The number of the hair cell of the third cochlear turn lost 111±55.8. After one week treatment with Dexamethason,the treated group's ABRT reduced to 48. 8±6. 69dBspl, the number of the outer—hair—cell of the third cochlear turn is only 57. 254±18. The results indicate that Dexamethon has effect on the AISNHL,it can clearly lighten the damage of inner ear tissue and significantly improve the ABRT. The mechanism still remains unknown.