摘要
采集成熟的泥鳅(Misgurnusanguillicaudatus)精子,在不同的渗透压、pH和温度条件下进行活力观察。结果表明:①保持泥鳅精子活力较强的适宜渗透压和pH分别是(100～200)mOsm(1mOsm≈0.248kPa)和pH7.0～8.0,而活力最强时的渗透压和pH则分别为150mOsm和7.6;②在(20～30)℃范围内泥鳅精子激活率均较高,且当温度为25℃时,其激活率最高;③泥鳅受精率最高时的最适渗透压和pH分别为150mOsm和7.6,这与泥鳅精子活力最强时的渗透压和pH条件保持高度一致。
The activation and vitality of spermatozoa from loach( Misgurnus anguillicaudatus ),which were collected by dissecting,were studied under different osmolality,pH and temperatures.The results were as follows:①The suitable osmolality and pH,which help preserve strong motility of the spermatozoa, are 100～200 mOsm and pH7.0～8.0,and the spermatozoa motility is the strongest under the conditions of 150 mOsm and pH7.6;②The suitable temperature for activating of spermatozoa is 20～30 ℃, and when temperature is 25 ℃,the activation rate is the highest;③The optimum osmolality and pH for the highest fertilization of loach are also 150 mOsm and 7.6 respectivly,consistent with the conditions which made the motility of the spermatozoa the strongest.
出处
《湖北农学院学报》
2004年第1期22-25,共4页
Journal of Hubei Agricultural College
关键词
渗透压
PH值
温度
泥鳅
精子活力
受精率
loach( Misgurnus anguillicaudatus )
sperm vitality
osmolality
pH
temperature
fertilization
