抗EB病毒EAp138单克隆抗体的研制及其在干燥综合征发病机制研究中的应用

Preparation of a Monoclonal Antibody Against EA p138 of Epstein-Barr Virus: Its Applicationin Sjogren's Syndrome

本研究将基因工程的成果与细胞工程(杂交瘤)技术相结合,研制了抗Epstein-Barr(EB)病毒早期抗原(early antigen,EA)p138单克隆抗体,特异性检查合乎要求。应用该特异性抗体对干燥综合征(Sjogren's syndrome,SS)和对照活检组织进行检测,结果:来自SS的4/13例泪腺,15/37例唇腺和所有7例肾脏组织呈阳性反应(免疫组化法和免疫荧光法),而对照标本均阴性。提示:EB病毒在SS的上述病变组织中处于活跃的复制生长期,其与SS的发病密切相关。

A monoclonal antibody against Epstein-Barr virus (EBV) encoded early antigen (EA) p138 was prepared with the use of a lysoprotein from the recombi-nant plasmid pUCARG1140. By using this specific monoclonal antibody and immunofluorescence as well as immunoenzymological methods, 4 of 13 lacrimal, 15 of 37 labial and all 7 renal biopsy tissues from patients with Sogren's syndrome (SS) were found to have positive staining of epithelial cells, whereas those of control persons were all negative. These results suggest that EBV has been in the stage of active replication in the aforementioned tissues of SS and this virus may have a close association with SS in terms of pathogenesis.