大鼠睾丸支持细胞、生精细胞及管周细胞的体外团聚研究

Studies on the Aggregation of Rat Sertoli Cells, Germ Cells and Peritubular Cells In Vitro

用5、10、15、20和25天龄大鼠睾丸曲细精管分离细胞制成悬液,在体外经固体琼脂培养和旋转培养,得到圆球状和索状细胞团聚体,其主要由支持细胞组成,管周细胞包绕在支持细胞外周。10天龄以前组,在团聚体中可见到部分生精细胞,15天龄以后组的生精细胞全部位于团聚体外.表明新生大鼠睾丸支持细胞、管周细胞及生精细胞间仍保留着部分胚胎期的识别和选择性粘附能力。

Dissociated testicular cell suspensions from 5, 10, 15, 20 and 25 day postnatal rats were cultured either on solid agar as a substratum or in a flask under slow rotation. Spherical or cord-like aggregations were formed from cells under both conditions. Sections from the aggregations showed that they were comprised mainly of Sertoli cells, with peritubular cells located around the aggregations. A few germ cells were present within those aggregations from cultures of rat testicular cells younger than ten days, while no germ cell could be found within aggregations from rats older than fifteen days. Based on these observations, we conclude that testicular cells in post-natal rats still maintain a limited capacity for cell adhesion and recognition and are thus able to form cellular aggregations.