花生区组间杂种二代性状鉴定

CHARACTERS IDENTIFICATION OF F_2 INTERSECTIONAL HYBRIDS IN PEANUT

花生区组四粒红与根茎区组Arachis glabrata杂交果针经离体培养克服了不亲和性,获得杂种。杂种二代性状出现疯狂分离,但有些性状仍分别倾向双亲。经叶片过氧化物同工酶分析,酶带的条数、宽窄、活性强弱及迁移率,杂种与双亲有明显差异,也有共同之处,初步证明了杂种的真实性。

By using aseptic peg culture technique,interspecific incompatibility between a peanut cultivar Silihong(Section Arachis)and A.glabrata(Section Rhizonmatosae)had been overcome,and a fertile hybrid was obtained,Although characters of FI hybrids exhibited a wide range of segregation,there were still some traits resemble to their parents.Leaf peroxidase isozyme PAGE analysis revealed that the hybrids were quite different ffom their parents in relation to band number,width,Rf value and isozyrne activity,but the zymograms of the hybrids and their parents were partially alike.This verified the authenticity of the hybrid nature.