荧光定糖法测定纤维素酶活力的条件研究

A Fluorescence Assay Method of Cellulase Activities

研究结果表明 ,荧光定糖法测定纤维素酶活力的反应最佳条件 :反应液浓度为46.7mg·g-1,反应时间为10min,反应温度为150℃。将荧光定糖法与滤纸酶活测定法相结合应用于纤维素酶蛋白组分及土壤纤维素酶活力的研究与测定。

For the measurement of low concentration enzyme,the filter paper activity and fluorescence assay methods were combined.The standard conditions for this method are as follows:The concentration of fluorescence reagent is46.7mg·g -1 ,the reaction temperˉature is150℃and the incubation time is10minutes.This method can solved the problem of cellulase protein isolation and can be used to determine cellulase activities of soils.