高效液相色谱法测定成人肠吸收试验中D-木糖的正常值

The measurement of D-xylose normal range of blood and urine of healthy volunteers using HPLC after D-xylose absorption testing

目的 :观察正常人肠吸收功能 ,用高效液相色谱法检测吸收试验中D 木糖血、尿中的正常值。 方法 :抽取5 0例健康人作木糖吸收试验 ,留取血和尿样。将乙腈与水 (88∶1 2 )作流动相 ,氨基柱作为分析柱 ,4 5min完成分离。 结果 :本试验建立血和尿的检测均具有良好的线性 ,相关系数分别为 0 .9998与 0 .9897,回收率为 (1 0 3.9±9.5 ) %与 (1 0 7.9± 1 0 .7) %。最低检测量可达 0 .0 1mmol/L ;此方法测出的健康人木糖吸收值 1h血清木糖水平为(2 .2 6± 0 .82 )mmol/L ;2h为 (2 .0 6± 0 .84 )mmol/L ;3h为 (1 .92± 0 .82 )mmol/L ;3h尿液排泄值为 (8.5 7±2 .70 )mmol/L ,5h为 (1 1 .33± 2 .98)mmol/L。 结论 :用高效液相色谱法检测D 木糖 ,方法简便 ,结果稳定可靠 ,是临床监测肠吸收功能的重要手段之一。

Objectives: To observe the function of small intestine absorption in healthy volunteer and to measure the normal range of D xylose concentrations in serum and urine using HPLC after D xylose testing. Methods: The serum and urine were sampled from 50 healthy volunteers after taking 5g of D xylose orally. Acetonitrile and water(88∶12) were used as moving phase, NH 2- column as analysis column, the isolation was finished in 45 minutes. Results: The levels of D xylose in serum and urine were well correlated with the peak value. The correlation coefficient were 0.9998 and 0.9897. The recovery rates were (103.9±9.5)% and (107.9±10.7)%. The low detection limit was 0.01 mmol/L. The normal values of D xylose in serum at 1,2 and 3 hour after testing were (2.26±0.82) mmol/L,( 2.06 ±0.84) mmol/L and (1.92±0.82) mmol/L, respectively. The urine D xylose concentrations for 3 and 5 hour after testing were (8.57±2.70) mmol/L and (11.33±2.98) mmol/L. Conclusions: It is simple and convenient for HPLC to measure the concentrations of D xylose in serum and urine after the D xylose absorption testing. The results are stable and credible.