川楝素腹腔注射对小白鼠半数致死量(LD_(50))的测定

Acute LD_(50 )determination of toosendanin in mice by ip injection

用寇氏 (k rber)法测定了川楝素腹腔注射对小鼠的半数致死量 (LD50 )。取 90只小鼠 ,雌雄各半 ,体重 (2 5± 2 )g。以 10 %丙二醇生理盐水为溶媒 ,按序贯法测出川楝素腹腔注射小鼠 10 0 %致死率 ;将高剂量组与低剂量组的组间剂量比设定为 1 2 ,染毒测定其LD50 。结果川楝素腹腔注射对小鼠的LD50 为 13 84mg/kg ,95 %可信限为 12 46～ 15 3 7mg/kg。

object: Using Karber's method to test the LD_(50 ) of Toosendanin in mice by ip injection . Method:taking 10% Propylene Glycol physiological saline as dissolvent for Toosendanin. 90 mice which half is male and another half is female was used to measure 100% lethal dose first, the rest was divided into 7 groups randomly. The ratio of high dose group and low dose group is set up as 1.2. Results showed that the LD_(50 ) values in this experiment is 13.84 mg/kg, with the 95% confidence limit of 12.4638～15.3701 mg/kg.