亚硒酸钠对大鼠肝癌细胞抑制作用的体外试验

Inhibition effects of sodium selenite on rat hepatoma cells in vitro

将不同质量浓度 (0 3、 0 5、 0 6、 0 8、 1 0、 1 2、 1 5和 2 0 μg·mL-1)的亚硒酸钠加入大鼠的肝癌细胞 (RH 35 )和正常肝细胞 (BRL)的体外培养液中 ,分别在不同时间 (2 4、 4 8、 72和 96h ;2 4、 5 6和 96h)检测肝癌细胞和正常肝细胞的活性、增殖能力、生长曲线等指标。结果表明 ,随着浓度和时间增加 ,亚硒酸钠对肝癌细胞的生长抑制作用逐渐增强 ;随时间的延长 ,亚硒酸钠对正常肝细胞的生长呈现一定的抑制作用 ,但浓度变化对正常肝细胞生长抑制的作用不明显。

The effect of sodium selenite in medium on cellular growth of cultured rat hepatoma cell line RH-35 and normal hepatic cell line BRL was investigated with concentration of 0.3,0.5,0.6,0.8,1.0,1.2,1.5 and 2.0 μg·mL -1 .The responses of the experimental cultured cells to the dosage and exposure time of selenium were analyzed by determining the viability of cells,the activity of cell multiplication and the cell survival curve at the exposure time of 24,48,72 and 96 h(rat hepatoma cell) and 24,56 and 96 h(normal hepatic cell).The results showed that various concentrations of sodium selenite could inhibit the growth of hepatoma cells at different time,whereas different concentrations of sodium selenite had no significantly effect on normal hepatic cell.But with the increasing of exposure time,sodium selenite also could inhibit the normal hepatic cells.