一株约翰逊不动杆菌的分离和鉴定

Identification of an isolate of Acinetobacter johnsonii

在土壤中分离到一株细菌STRB ,该菌可以在SFM、LB、EMB、PDA、MM、MacC等多种培养基上生长 ,革兰氏染色为阴性 ,电镜观察无鞭毛。生化指标检测显示 ,该菌株对多数糖类不能发酵 ,经bioMerieuxVitek公司的革兰氏阴性细菌检测试验卡 (GNI)鉴定为醋酸钙不动杆菌。设计 2对简并引物对其 16SrRNA进行PCR扩增 ,16SrRNA序列及其进化树分析结果显示 ,该菌为约翰逊不动杆菌。

A bacterial strain named STR B was isolated from soil,which could be cultivated on some media,such as SFM,LB,EMB,PDA,MM and MacC agar.It was gram negative and could be viewed without flagellas under electron microscope.The strain could not ferment most of sugars in the biochemical test and it was identified as Acinetobacter calcoaceticus with the gram negative identification card(GNI)of bioMerieux Vitek Company.Two pairs of primers were designed for PCR of its 16S rRNA.The sequence analysis results of 16S rRNA sequence with online software showed that the STR B belonged to Acinetobacter johnsonii,not a strain of Acinetobacter calcoaceticus.