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不同预处理对小麦幼胚抗除草剂基因转化效率的影响 被引量：1

Effect of different pre-treatment on the frequency of transformation of herbicide-resistant gene of wheat embryo
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摘要 以小麦品种豫麦18号和豫麦34号作为供试材料,用基因枪介导法进行了抗除草剂基因的转化,研究了不同预培养时间和高渗处理对小麦幼胚转化效果的影响.结果表明,幼胚以诱导培养10～15d的转化效果最好;用0 4mol·L-1的甘露醇在轰击前6h和轰击后18h对10～15d预培养的幼胚进行处理,可明显提高转化效率. Two wheat cultivars,Yumai 18 and Yumai 34 were used as experiment materials to study the effect of different pre-culture duration of immature embryo and osmotic treatment on tranformation frequency by transforming herbicide-resistant gene through particle baombardment.The result showed that the immature embryos pre-cultured for 10～15 days and treated with 0.4 mol·L^(-1) mannitol could significantly improve gene transformation frequency.
作者 任江萍 王新国 卫丽 尹钧 师学珍 李磊
机构地区 河南农业大学国家小麦工程技术研究中心 河南师范大学
出处 《河南农业大学学报》 CAS CSCD 2004年第2期127-130,共4页 Journal of Henan Agricultural University
基金 河南省杰出人才创新基金项目(12100800)
关键词 预处理 小麦 幼胚 抗除草剂基因 基因转化 转化效率 基因枪 wheat pre-treatment herbicide-resistant gene particle gun
分类号 S512.1 [农业科学—作物学]
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参考文献10

  • 1BLIFFELD M, MUNDY J, POTRYKUS I, et al. Genetic engineering of wheat for increased resistance to powdery mildew disease[ J]. Theor Appl Genet, 1999,98: 1079 - 1086.
  • 2FERDY A, DIAZ I, MCAUSLANE H, et al. Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor Cme[J]. Molecular Breeding, 1999,5: 53 - 63.
  • 3ZHANG L, RYBCXYNSKI J J, LANGENBERG W G, et al. An efficient wheat transformation procedure:transformed calli with longterm morphogenic potential for plant regeneration [ J ]. Plant Cell Reports, 2000,19: 241 - 250.
  • 4MURASHIGE T, SKOOG F. A revised medium for rapid growth and bioassays with tobacco tissue cultures[ J ]. Plant Physiol, 1962,15: 473 - 497.
  • 5SHORT J M, FERNANDEZ J M, SORGE J A, et al. λ zap:A bacteriophage λ expression vactor with vivo excision properties [ J ]. Nucleic Acids Res, 1988,16:7583.
  • 6VASIL V, SRIVASTAVA V, CASTILLO A M, et al. Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos[ J]. Bio/Technology, 1993,11:1553 - 1558.
  • 7TAKUMI S, SHIMADA T. Production of transgenic wheat through particle bombardment of scutellar tissues: frequency is influenced by culture duration[ J]. J. Plant Physiol, 1996,149:418 - 423.
  • 8FREDY A, VASIL V, SRIVASTAVA V, et al. Accelerated production of transgenic wheat ( Triticum aestivum L. ) plants [ J ]. Plant Cell Reports, 1996,16:12 - 17.
  • 9VAIN P, MICHAEL D M, FINER J J. Treatment enhances particle bombardment-mediated transient and stable transformation of maize[J]. Plant Cell Reports, 1993,12 (2): 84 - 88.
  • 10ROCHANGE F, SERRANO L, MARQUE C, et al. DNA delivery into Eucalyptus zygotic embryos through biolistics:optimization of the biological and physical parameters of bombardment for two different particle guns[ J ]. Plant Cell Reports, 1995,14:674 - 678.

同被引文献25

  • 1杨剑波,许智宏,卫志明,白永延.影响根癌农杆菌附着禾谷类作物培养细胞的因素[J].实验生物学报,1993,26(1):1-9. 被引量：17
  • 2王艳丽,叶兴国,刘艳鹏,杜丽璞,徐惠君.农杆菌敏感小麦基因型的筛选研究[J].麦类作物学报,2005,25(6):6-10. 被引量：24
  • 3Shou H X, Frame B R, Whitham S A, Wang K. Assessment of transgenic maize events produced by particle bombardment or Agrobacterium-mediated transformation. Mol Breed, 2004, 13: 201- 208
  • 4Cheng M, Fry J E, Pang S, Zhou H, Hironaka C M, Duncan D R, Conner T W, Wan Y. Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiol, 1997, 115:971-980.
  • 5Dai S, Zheng P, Marmey P, Zhang S, Tian W Z, Chen S Y, Beachy R N, Fau C. Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Mol Breed, 2001, 7:25-33.
  • 6Fagard M, Vaucheret H. Transgene siliencing in plants: how many mechanisms? Annu Rev Plant Physiol Plant Mol Biol, 2000, 51 : 167-194.
  • 7Hiei Y, Ohta S, Komari T, Kumasho T. Efficient transformation of rice ( Oryza sativa L. ) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J, 1994, 6:271-282.
  • 8Vaucheret H, Fagard M. Transcriptional gene silencing in plants: targets, inducers and regulators. Trends Genet, 2001, 17:29-35.
  • 9Zhao Z Y, Gu W, Cai T, Tagliani L A, Hondred D A, Bond D, Krell S, Rudert M L, Bruce W B, Pierce D A. Molecular analysis of To plants transformed by Agrobacterium and comparison of Agrobacteriummediated transformation with bombardment transformation in maize. Maize Genet Coop Newsl, 1998, 72: 34- 37.
  • 10Ishida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T. High efficiency transformation of maize (Zea mays L. ) mediated by Agrobacterium tumefaciens. Nat Biotech, 1996, 14:745-750.

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河南农业大学学报
2004年 第2期

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