抗乙酰胆碱受体单克隆抗体A7诱导实验性重症肌无力

Effect of anti-acetylcholme receptor monoclonal antibody A7 in experimental autoimmune myasthenia gravis

目的:探讨抗乙酰胆碱受体单克隆抗体A7(Anti-AChR mAb A7)对实验性自身免疫性重症肌无力(EAMG)的作用。方法:Lewis大鼠腹腔注射5 ml 20倍浓缩的含Anti-AChR mAb A7培养上清液,建立EAMG被动转移模型。对照组腹腔注射同体积的PBS。每8 h监测体重和临床症状评分。48 h后处死实验动物。用放射免疫法检测AChR,并计算出AChR的损失率。结果:Anti-AChR mAb A7诱导的EAMG模型AChR损失率高,为24.3％-45.2％。而且AChR损失率与临床症状评分相关(r=0.74,P<0.01)。Anti-AChR mAb A7攻击的位点在终板的AChR上。结论:Anti-AChR mAb A7可用作诱导EAMG模型而应用于重症肌无力的研究。

Objective:To investigation the effect of anti-acetylcholine receptor monoclonal antibody A7 in experimental autoimmune myasthenia gravis (EAMG) . Methods: Passive transfer EAMG model was induced by i. p. injection of 20 fold concentrated culture supernatant of A7 in final volume 5 ml in lewis rats. Control rats were injected with 5 ml PBS. Monitor body weight and assessment clinical signs score each eight hours. Animal were killed 48 hours after injection of the mAb A7. Loss of acetylcholine receptor (AChR) was confirmed by measurement of the muscle AChR concentration with radioim-munoassay (RIA) in whole casses and calculated the AChR loss rate. Results: AChR loss rate were higher in EAMG induced by mAb A7,from 24.3% to 45.2%,and it is correlated to clinical signs score (r = 0.74, P<0.01). The target site of mAb A7 is in AChR of end-plate. Conclusion: Mab A7 can be used to induce EAMG as a model to investigate the pathogenesis of myasthenia gravis.