体外厌氧条件下载铜蒙脱石杀菌效果的研究

ANTIBACTERIAL EFFECT OF COPPER-MONTMORILLONITE ON ORAL BACTERIA UNDER ANAEROBIC CONDITIONS

载铜蒙脱石的杀菌作用通过脑心浸液肉汤 (BHI)二倍系列稀释法来判定其最小抑菌浓度 (MIC)。释放入肉汤和生理盐水中Cu2 +的量通过原子吸收光谱仪测定。杀菌动力学研究采用改良的振荡瓶法。结果表明 :厌氧条件下 ,载铜蒙脱石具有很强的杀菌性能。载铜蒙脱石对放线共生放线杆菌的最小抑菌浓度为 2 .5 6mg/ml;对血液链球菌为 5 .12mg/ml。测得肉汤二倍稀释的载铜蒙脱石释放出的Cu2 +量在 2 .39～ 38.6 5 ppm之间 ,在生理盐水中释放的Cu2 +量为 1.85～ 15 .82ppm。本试验结果表明 ,蒙脱石无抗菌性能。

The antibacterial activity of copper-montmorillonite(MMT-Cu) was evaluated by measuring the minimum inhibitory concentrations (MICs) using two-fold serial dilutions of MMT-Cu in Brain Heart Infusion Broth (BHI). Release of Cu 2+ into the broth was measured by atomic absorbent spectrometry. Study of kinetics on antibacterial was conducted by using a modified method of vibrate flask. Results show that MMT-Cu inhibited the growth of bacteria tested under the anaerobic conditions. The MIC of MMT-Cu is 2.56mg/ml on Actinobacillus actinomycetemcomitans, and 5.12 mg/ml on Streptococcus sanguis. The concentration of Cu2+ released into the broth rangs from 2.39 to 38.65ppm; The concentration of Cu2 + releasing into saline rangs from 1.85 to 15.82ppm. Results also show that MMT has no antibacterial activity.