低浓度Zn对幼龄鲫鱼肝脏组织应激蛋白HSP70诱导的影响

Western Blotting Responses of Heat Shock Protein (HSP70) in the Liver of Young Fish, Carassius Auratus to Lower Concentration of Zinc (Zn2+ )

选择鲫鱼(Carassiusauratus)作为受试生物,经过40dZn2+不同浓度的暴露后,运用SDS-PAGE和WesternBlot-ting方法检测了鱼肝脏组织内应激蛋白HSP70的诱导表达情况。结果表明,在各试验浓度下,与对照组相比,Zn2+对鱼肝脏内HSP70有显著的诱导(P<0.05)。试验中还发现,在试验浓度低于国家渔业用水标准时,HSP70相对于对照组仍然表现为明显诱导(P<0.05),充分说明分子生物学指标要比传统的环境检测指标敏感,对污染物早期具有预警的作用。

The Effects of Zinc (Zn2+ ) on the induction of HSP70 in the fish liver were studied. After the fish (Carassius auratus) were exposed at different Zinc (Zn2+ ) concentrations for forty days, the HSP70 in the fish liver was measured using the SDS- PAGE and Western Blotting technique. Compared with those under the control, the groups exposed to the Zinc (Zn2+ ) all showed a significant increase in HSP70 as a function of exposure (P< 0.05). The fish exposed to the concentration of Zn2+ below the national fishery water quality standard also had a significant increase in HSP70, which implied that the low concentration of the heavy metal also could harm the fish body. HSP70 as a environmental biomarker could be a more sensitive and rapid indicator than conventional end points such as the growth death.