三种氨基甲酸酯类农药化合物对DNA的潜在损伤作用

The Potential Trauma of Three Kinds of Carbamate Pesticides to DNA

应用紫外光谱法研究了3种氨基甲酸酯类农药对DNA的损伤作用。结果显示,这3种氨基甲酸酯类农药能够引起ctDNA的紫外吸收光谱发生发射波长以及吸收强度的变化,从而说明3种氨基甲酸酯类农药能够与ctDNA进行作用。即意味着3种氨基甲酸酯类农药进入生物有机体后有可能通过形成DNA加合物的形式而进一步导致基因突变,最终对生物体的DNA产生化学损伤。

DNA is the main germplasm of most organisms. The characters of organisms are just the expression of it. And it also takes charge of the passing of biologic information to assure the stability of the biologic races. So it will be of importance to keep DNA to be steady, which can maintain some continuity among races. However, some chemical compounds or their metabolic products in the environment, being electrophilic, inclined to bind to the lone- pair electrons of the bases of DNA to produce DNA adducts. And it is well known that DNA adducts is the prelude of gene mutation, which means that DNA adducts can result in the changes of the information of DNA if the repairing of the organism can not resume the changes. So DNA adducts can be used to forecast the biologic safeties of the chemical compounds used. Some experiments have shown that the chemical compounds can react with DNA outside, and if that can happen in the bodies of the organism itself, it can predigest the tests to save a lot of times, manpower and material resources.Pesticides usually used to control the pest for a good harvest may lead to some leftovers, which may be transferred to the organism by the means of ecosystem. So the leftovers may have the chance of binding to the DNA to form the DNA adducts, which will be dangerous to the animals and human being. The biologic activities of three kinds of pesticides, diethofencarb, carbaryl and carbofuran were tested. They all assumed white colors, had different solubilities with 26.5 μ g· mL- 1, 40 μ g· mL- 1 and 700 μ g· mL- 1, and according to their activities, they belonged to bactericide, pesticide and pesticide, respectively.Using UV spectrum, the effects of the three kinds of carbamate pesticides on DNA was studied. The carbamate pesticides changed the positions and intensions of absorbing peaks, which implied that they could bind to ct DNA to produce DNA adducts. The carbamate pesticides might result in the gene mutation and bring the chemical trauma to the DNA of the organisms through producing DNA adduts.