食管癌细胞中c-erbB-2与P-gp表达的相关性

Relationship between c-erbB-2 and P-glycoprotein expression in esophageal carcinoma

目的 探讨食管癌细胞c erbB 2与多药耐药性相关性及其临床意义。方法 用免疫组化方法检测癌细胞c erbB 2和用流式细胞术检测了 4 6例食管癌手术标本中癌细胞P 糖蛋白 (P gp)表达水平 ,并进行相关性分析。 结果 食管癌中c erbB 2表达阳性 12 / 4 6例 (2 6 1% ) ;P gp表达阳性 2 8/ 4 6例 (6 0 9% )。食管癌淋巴结转移情况与c erbB 2和P gp表达比较发现 ,在c erbB 2阳性时淋巴结有转移 (31 0 9%± 5 33% )与无转移 (8 0 4 %± 3 0 3% )组的P gp表达比较差异有显著性(P =0 0 0 73) ,而在c erbB 2阴性时淋巴结转移情况与P gp表达无相关性。食管癌临床分期与c erbB 2和P gp表达比较发现 ,在c erbB 2阳性时 ,Ⅲ～Ⅳ期 (33 6 8%± 5 5 1% )与Ⅱ期 (9 30 %± 2 78% )组P gp表达比较 ,差异有显著性 (P =0 0 0 2 7) ,而在c erbB 2阴性时临床分期与P gp表达无相关性。食管癌肿瘤大小、肿瘤分化程度与c erbB 2和P gp表达均无相关性。结论 在c erbB 2表达阳性时食管癌中有淋巴结转移、临床Ⅲ～Ⅳ期患者与P gp表达升高有相关性 ,提示双阳性时肿瘤患者预后较差。但在c erbB 2表达阴性时食管癌有淋巴结转移、临床分期与P

Purpose To investigate the relationship between c-erbB-2 and multidrug resistance (MDR) as well as its clinical significance. Methods To examine the expression level of c-erbB-2 by immunohistochemistry assay and at the same time to detect the level P-glycoprotein (P-gp) by flow cytometry in the operating samples from 46 cases with esophageal carcinoma. Results C-erbB-2 positive expression was found in 12/46 (26.1%) cases with esophageal carcinoma. The P-gp positive expression was found in 28/46 (60.9%). In c-erbB-2 positive tumors, significant differences of P-gp expression between the group with lymphnode involvement (31.09%±5.33%) and the one without node metastasis (8.04%±3.03%), and between the groups at stage Ⅲ～Ⅳ (33.68%±5.51%) and the one at stage Ⅱ (9.30%±2.78%) was found, while in c-erbB-2 negative tumors, no signifficant differences was found. The tumor size and differentiation were not related to c-erbB-2 and P-gp expression. Conclusions The P-gp expression at high level is related to the positive expression of c-erbB-2 and lymph node metastasis in clinical Ⅲ～Ⅳ stage patients with esophageal carcinoma. The results also suggest that the double positivity might be a poor prognosis.