双氯灭痛对豚鼠表皮角质形成细胞/黑素细胞比值及前列腺素E_2含量影响的实验观察

Effect of diclofenac sodium on KC/MC ratios and content of PGE_2 in epidermis of the full-thickness skin autografts

目的 研究双氯灭痛对豚鼠皮肤前列腺素E2 含量变化及自体全厚皮片表皮内角质形成细胞 /黑色素细胞 (KC/MC)比值与色素沉着的影响。方法 借助放射免疫分析方法动态观察皮片内前列腺素E2 (PGE2 )含量的变化及双氯灭痛的影响 ;借助组织学及组织化学方法观察皮片表皮内KC/MC比值变化。结果 在手术对照组 ,移植早期PGE2 含量升高伴随着KC/MC比值的升高 ;而实验后期KC与MC的比值 (KMR)减小。在双氯灭痛组 ,早期PGE2 不升反降 ,KMR减小 ;相应的实验后期KMR增大 ,皮片色泽较浅。结论 在移植早期应用双氯灭痛可以减轻炎症反应对表皮黑色素单位的损伤 ,减弱其增殖反应 ,从而降低KMR ,减轻皮片色素沉着的程度。

Objective To study effect of diclofenac sodium on content of prostaglandin E 2 KC/MC ratio and hyperpigmentation of the full-thickness skin autografts. Methods Content of PGE 2 was determined by radioimmune analysis method, and kinetic changes of KC/MC ratio were observed with histological and histochemical methods. Results In the operation control group, increase of KC/MC ratios was accompanied by of PGE 2 content at the early stage of the experiment; and the KMR became smaller than that in normal control group at the end of the experiment. While in the diclofenac soduim group, KMR was reduced due to the less damage of MC at the early stage, with decrease of PGE 2. Accordingly, KMR became larger at the late period of the experiment, and color of the autografts became lighter. Conclusion Application of diclofenac sodium on the grafts just after transplantation may reduce the damage of inflammation to the epidermal melanin units, control their proliferation and decrease the value of KMR, which lightens the color of the grafts.