高压氧对脑缺血再灌注大鼠的海马诱导型一氧化氮合酶mRNA表达的影响

Effects of hyperbaric oxygen on expression of inducible nitric oxide synthase mRNA in hippocampus of rats after cerebral ischemia-reperfusion

目的 观察高压氧对脑缺血再灌注大鼠的海马诱导型一氧化氮合酶信使核糖核酸 (iNOSmRNA)表达的影响。方法 将 5 4只大鼠随机分为脑缺血再灌注组 (IR组 )、脑缺血再灌注加高压氧处理组(HBO组 )和对照组 ,建立脑缺血再灌注大鼠动物模型。应用荧光定量逆转录 聚合酶链反应 (RT PCR )技术检测各组在再灌注 6h、2 4h、48h、96h时相点大鼠的海马iNOSmRNA表达。结果 再灌注各时相点的IR组和HBO组的海马iNOSmRNA表达均显著高于对照组 (均P <0 .0 1) ;HBO组大鼠再灌注 2 4h、48h、96h时海马iNOSmRNA表达均显著低于IR组 (P <0 .0 5 ,P <0 .0 1,P <0 .0 1)。结论 高压氧治疗可以显著抑制再灌注后大鼠海马iNOSmRNA表达 ,从而减少延迟性NO的产生 ,有助于减轻脑缺血再灌注损伤。

Objective To investigate the effects of hyperbaric oxygen(HBO) on expression of inducible nitric oxide synthase(iNOS) mRNA in hippocampus of rats after cerebral ischemia-reperfusion. Methods The rats were randomly divided into a cerebral ischemia-reperfusion group (CIR group), a hyperbaric oxygen group (HBO group) and a sham-operation group(SO group). The cerebral ischemia-reperfusion models were established. The expression of iNOS mRNA in the hippocampus of rats was measured at 6h, 24h, 48h and 96h, respectively, after reperfusion by using fluorescent quantitative reverse transcription PCR (RT-PCR). Results The expression of iNOS mRNA in hippocampus in CIR and HBO groups were significantly higher than those in the SO group at 6h, 24h, 48h, 96h (P<0.01) after reperfusion. The expression of iNOS mRNA in hippocampus of rats in the HBO group were significantly lower than those in the CIR group at 24h, 48h, 96h after reperfusion (P<0.05,P<0.01, P<0.01, respectively). Conclusion HBO treatment can effectively relieve the cerebral ischemia-reperfusion injury through inhibiting the expression of iNOS mRNA in hippocampus of rats after cerebral ischemia-reperfusion and decrease the production of NO.