性激素在急性肺损伤中作用的初步研究

Study on the function of sex hormone in acute lung injury

目的 探讨性激素在大鼠急性肺损伤中对肺脏病理变化的可能作用及机制。方法 复制大肠杆菌内毒素脂多糖诱发的雄性大鼠急性肺损伤的模型 ,切除性腺 15d后予以补充雌二醇 (1mg/kg) ,观察急性肺损伤大鼠的肺组织显微病理变化 ,并应用免疫组化技术测定肺组织中雌激素受体、孕激素受体的表达及应用放免法测定外周血中的雌二醇、催乳素浓度变化。结果 雌二醇治疗组 (A组 )大鼠肺组织病理损伤较睾丸切除组 (B组 )大鼠为轻 ,B组大鼠肺组织病理损伤较假睾丸切除组 (C组 )大鼠为轻 :A组与B、C组及正常对照组 (D组 )相比外周血中的雌二醇及催乳素的浓度明显升高 ,差异有显著性 ,B组较C组及D组外周血中的雌二醇浓度下降 ,差异有显著性 ;A组雌激素、孕激素受体的阳性表达率较B、C、D组升高 ,差异有显著性。结论 高雌激素水平对脂多糖诱导的急性肺损伤有保护作用 ,而低雄激素、高催乳素水平可能是对脂多糖诱导的急性肺损伤起辅助性保护性作用。

Objective To explore the possible pathologic effects of sex hormone on acute lung injury.Methods In rats with acute lung injury (ALI) induced by escherichia coli lipopolysaccharide,using immunohistochemistry and radio-immunoassay, we observed pathological changes after gonad resection with estradiol supplementation, measured estrogen receptor and progesterone receptor in rat lung as well as estradiol and prolactin in rat peripheral blood.Results The pathological lung lesions in group A,which given estradiol,significantly milder than that in group B, which only had gonad resected. And the lesion in group B was much milder than that in group C, which was had gonad pseud oresected. Compare with group B, group C and group D, the concentration of estradiol and prolactin in rat peripheral blood in group A were significantly higher (P <0.01 ) . Compare with group C and group D, the concentration of estradiol and prolactin in rat peripheral blood in group B is significantly lower (P<0.05) .The expressions of estrogen receptor and progesterone receptor in rat lung in group A were significantly higher that that in group B, group C or group D (P<0.05 ) .Conclusion Low androgen, high estrogen and high prolactin can protect the LPS-induced ALI.