肝细胞癌细胞周期蛋白D1基因的扩增和表达

A study of gene amplification and expression of cyclin D1 in hepatocellular carcinoma

目的 探讨细胞周期蛋白 (cyclin)D1基因 (CCND1)扩增和cyclinD1在肝细胞癌 (HCC)中的表达情况及其与HCC发生发展的关系。方法 分别应用差异聚合酶链反应 (DPCR)、逆转录(RT) PCR和免疫组织化学法检测 2 0例HCC中cyclinD1基因扩增率、mRNA和蛋白表达状况 ,并分析其与HCC组织学分级的关系。结果 cyclinD1基因扩增结果为 6/ 2 0 ,其mRNA和蛋白过表达分别为9/ 2 0和 14 / 2 0。cyclinD1mRNA表达与基因扩增相关 (P <0 .0 5) ,也和蛋白表达水平相关 (P <0 .0 5)。cyclinD1蛋白表达与HCC组织学分级具有相关性 (P <0 .0 5)。结论 cyclinD1基因扩增在HCC中较常见 ,是引起cyclinD1mRNA和蛋白过表达的主要原因之一。cyclinD1蛋白过表达与HCC的组织学分级相关。

ObjectiveTo investigate gene amplification of CCND1 and expression of cyclin D1 in hepatocellular carcinoma (HCC) and to explore the possible relationship between CCND1 gene status and carcinogenesis of HCC. MethodsDifferential PCR, RT-PCR and immunohistochemistry were used to detect gene amplification, mRNA and protein expression of cyclin D1 in 20 HCC cases respectively. The relationship between the gene amplification rate and the expression level of cyclin D1 and the histological grades of HCC was analyzed. ResultsCCND1 gene amplification was detected in 30% of the cases HCC. An overexpression of cyclin D1 mRNA and protein could be demonstrated in 45% and 70% cases respectively. The expression of cyclin D1 mRNA correlated with its gene amplification status (P<0.05) and was responsible for the protein expression level (P<0.05). There was a close relationship between the expression level of cyclin D1 protein and HCC histological grades (P<0.05). ConclusionsCCND1 gene amplification is a common phenomenon in HCC and may be directly responsible for the cyclin D1 mRNA and protein overexpression. Cyclin D1 protein expression level is directly related to HCC histological grades. Therefore, CCND1 amplification and cyclin D1 overexpression may play an important role in development and differentiation of HCC.