HSP70,Bcl-2和PCNA在鼻咽癌中的表达及其意义

Study on the expression of HSP70,Bcl-2 and PCNA in nasopharyngeal carcinoma and clinical significance

目的 探讨HSP70 ,Bcl 2和PCNA在鼻咽癌中的表达及临床意义。方法 用免疫组化S P法分别检测HSP70 ,Bcl 2和PCNA在 6 0例鼻咽癌组织中的表达。结果 ①HSP70 ,Bcl 2和PCNA在鼻咽癌组织中的阳性表达率分别为 70 % (4 2 / 6 0 )、6 5 % (39/ 6 0 )和 86 .7% (5 2 / 6 0 )。②HSP70表达阳性和阴性病人的预后组间无统计学意义 (P >0 .0 5 ) ;Bcl 2、PCNA表达阳性和阴性病人以及不同临床时期 (Ⅱ、Ⅲ、Ⅳ )病人的生存状态比较有统计学意义 (P <0 .0 5 )。③鼻咽癌组织中HSP70和Bcl 2的表达一致率为 75 % (4 5 / 6 0 ) ,存在统计学意义 (P <0 .0 5 ) ,PCNA与Bcl 2、HSP70间无显著相关。④HSP70、PCNA阳性表达在鼻咽癌不同临床时期 (Ⅱ、Ⅲ、Ⅳ )比较有统计学意义 (P <0 .0 5 )。结论 HSP70表达与鼻咽癌临床分期相关 ,但可能并不直接反映预后 ;Bcl 2、PCNA表达在鼻咽癌预后评估中有一定意义。

Objective To investigate heat shock protein 70 (HSP70), Bcl-2 and proliferation cell nuclear antigen (PCNA) expression in nasopharyngeal carcinoma (NPC) and clinical significance, and analysize their relations. Method HSP70, Bcl-2 and PCNA expression were andlysed respectively in 60 nasopharyngeal carcinoma tissue by immunohistochemical method. Results ① the positive expression rates of HSP70, Bcl-2 vs PCNA in NPC were 70% (42/60)?65% (39/60) and 86.7% (52/60) respectively. ② the mean survival period, the five-year-survival rate and median survival period in Bcl-2 and PCNA positive expression group were shorter than Bcl-2 and PCNA negative expression group (P< 0.05); there is also significant difference among different stages of NPC as to the survival condition; but no significant survival difference showed between HSP70 positive group and negative group. ③ the coincidence rate of HSP70 and Bcl-2 expression was 75% (45/60) and significant correlation showed between HSP70 and Bcl-2 (P< 0.05), no significant correlation existed between HSP70 and PCNA, PCNA and Bcl-2. ④ There is significant difference of HSP70?PCNA expression in stage Ⅱ?Ⅲ?Ⅳ of NPC (P< 0.05). Conclusion HSP70 expression could reflect clinical stage of NPC, but was not significant in survival of NPC patients; Bcl-2?PCNA expression may be a useful prognosis marker in NPC.