高渗法制备过程中红细胞及其吗啡载体的形态学

Morphological observation of erythrocyte during the preparation of morphine carrier by a hyperosmotic method

目的 了解高渗法制备吗啡红细胞载体过程中红细胞形态及体积的变化规律,为阐明红细胞药物载体的载药机制提供依据。方法 采用自动化血细胞分析仪及倒置显微镜、扫描电子显微镜等技术,对正常红细胞、脱水红细胞及载药红细胞的体积、体积分布宽度、直径、厚度、外观等指标进行观测,比较载体形成前后红细胞的形态学变化。结果 经高渗脱水的红细胞的体积较正常的缩小18.7％,载药红细胞的体积分别较正常及脱水红细胞增大20.4％和49.8％;载药红细胞趋球形状态,脱水红细胞则趋扁平状态;有一定量的脱水红细胞呈空泡状态;部分脱水红细胞表面皱缩,当其形成载药红细胞时,皱缩现象基本消失。结论 利用红细胞在高渗液中脱水在低渗药液中膨胀的原理,制备红细胞药物载体。

OBJECTIVE: To describe the morphologic changes of erythrocyte during the preparation of morphine carrier by a hyperosmotic method, and try to illustrate the mechanism of erythrocyte carrier encapsulating drugs. METHODS: Automated hemocyte analysis meter, inverted microscope and scaning microscope were used to measure mean cell volume (MCV), volume distribution width (VDW), diameter, thickness and external appearance of erythrocyte and its morphine carrier prepared by a hyperosmotic method. The morphologies before and after carrier formation were compared. RESULTS: MCV of dehydrated-RBCs was 18.7% less than that of native RBCs, while MCV of encapsulated-RBCs was 20.4% and 49.8% bigger than that of native and dehydrated RBCs. Encapsulated-RBCs exhibited a tendency toward spherical shape and dehydrated-RBCs exhibited a tendency toward flat shape. Vacuolization was found in a few dehydrated-RBCs. Shrinkage occured in the surface of partial dehydrated-RBCs, and disappeared when dehydrated-RBCs changed into encapsulated- RBCs. CONCLUSION: Preparation of carrier erythrocyte carrier encapsulating drugs was based on a principle that erythrocytes were dehydrated in hyperosmotic solutions and expanded in hypotonic solutions.