摘要
Background Generally speaking, undifferentiated keratinocytes may synthesize a larger amount of IL-α and its production is decreased as cells complete differentiation gradually. Ultraviolet B (UVB) light can signigicantly stimulate the production and release of some cytokines. In this study we investigated the influence of UVB irradiation on IL-1α and adenosine triphosphoric (ATP) mRNA expressions in the human keratinocyte (KC) of original squamous cell carcinoma line (SCC 12F cells).Methods The cultured SCC 12F cells were irradiated with 30 mJ/cm 2 of UVB. Northern blot was employed to analyze the expression of IL-1α and ATP mRNA. Results There was a constitutive expression of IL-1α mRNA in SCC 12F cells. The expression increased in culturing time in regular KC medium and reached the highest expression at 120 hours. The expression level of IL-1α was up-regulated with two peaks at 6 hours and 72 hours, respectively after UVB irradiation. In comparison with IL-1α mRNA expression, ATP mRNA was down-regulated, with similar biphasic peaks, compared with the sham irradiated group. Conclusions SCC12F cells may express IL-1α mRNA constitutively. After UVB irradiation, the mRNA expression of IL-1α and ATP will show opposite effect because of inflammation/immunity and energy consumption mechanisms.
Background Generally speaking, undifferentiated keratinocytes may synthesize a larger amount of IL-α and its production is decreased as cells complete differentiation gradually. Ultraviolet B (UVB) light can signigicantly stimulate the production and release of some cytokines. In this study we investigated the influence of UVB irradiation on IL-1α and adenosine triphosphoric (ATP) mRNA expressions in the human keratinocyte (KC) of original squamous cell carcinoma line (SCC 12F cells).Methods The cultured SCC 12F cells were irradiated with 30 mJ/cm 2 of UVB. Northern blot was employed to analyze the expression of IL-1α and ATP mRNA. Results There was a constitutive expression of IL-1α mRNA in SCC 12F cells. The expression increased in culturing time in regular KC medium and reached the highest expression at 120 hours. The expression level of IL-1α was up-regulated with two peaks at 6 hours and 72 hours, respectively after UVB irradiation. In comparison with IL-1α mRNA expression, ATP mRNA was down-regulated, with similar biphasic peaks, compared with the sham irradiated group. Conclusions SCC12F cells may express IL-1α mRNA constitutively. After UVB irradiation, the mRNA expression of IL-1α and ATP will show opposite effect because of inflammation/immunity and energy consumption mechanisms.
