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四种方法检测超广谱β-内酰胺酶的敏感性比较

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摘要 目的 比较 4种方法检测超广谱 β -内酰胺酶 (ESBLs)的敏感性。 方法 用纸片扩散初筛法从临床分离的 1 0 5株肠杆菌科细菌筛选出可疑产ESBLs的菌株 ,同时用纸片扩散确证法、双纸片协同法、浓度梯度法(Etest)和Vitek32型全自动分析系统药敏检测卡GNS - 5 0 6进行ESBLs的检测。结果 共检出ESBLs菌株 4 2株。双纸片协同法、浓度梯度法 (Etest)和Vitek -GNS - 5 0 6三者的敏感性分别为 1 0 0 0 % ,81 0 % ,85 7% ,特异性均为1 0 0 0 %。结论 纸片扩散确证法简便、准确 ,适用于各级医院常规应用。
出处 《广东医学》 CAS CSCD 2004年第5期521-523,共3页 Guangdong Medical Journal
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  • 1National Committee for Clinical Laboratory Standards. Perfopmance standards for antimicrobial susceptibility testing; ninth information. Standard M100 - S 9. Pennsylvania: NCCLS, 1999.1 - 1033
  • 2Sanders CC, Barry AL, Washington JA, et al. Detection of extended spectrum - beta - lactamase - producing members of the family Enterobacteriaceae with Vitek ESBL test. J Clin Microbiol, 1996,34:2997
  • 3Vercauteren E, Descheemaeker P, leven M, et al. Comparison of screening methods for detection of extended - spectrum beta - lactamases and their prevalence among blood isolates of Escherichia coli and Klebsiella spp. in a Belgian teaching hospital. J Clin
  • 4Tzelepi E, Giakkoupi P, Sofianou D, et al. Detection of extended - spectrum beta - lactamases in clinical isolates of Enterobacter cloacae and Enterobacter aerogenes. J Clin Microbiol, 2000,38: 542
  • 5Midolo PD, Matthews D, Fernandez CD, et al. Detection of extended spectrum beta- lactamases in the routine clinical microbiology laboratory.Pathology, 2002,34: 362
  • 6Coudron PE, Moland ES, Sanders CC. Occurrence and detection of extended - spectrum beta - lactamases in members of the family Enterobacteriaceae at a veterans medical center: seek and you may find. J Clin Microbiol, 1997,35:2593

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