低聚异麦芽糖产品中主要组分的毛细管电泳分析方法

Determination of Main Components of Isomaltooligosaccharide Fomulation by Capillary Electrophoresis

在未涂渍的石英毛细管中 ,以 75mmol/L硼砂 (pH 1 0 5 )为运行电解质 ,在检测波长 2 1 4nm、分离电压 1 5kV下对低聚异麦芽糖的主要组分的α 萘胺衍生物进行了快速分离测定。结果表明 ,该方法重现性好 ,迁移时间和峰面积的相对标准偏差分别在 0 5 %和 2 0 %以内。异麦芽糖、异麦芽三糖、潘糖浓度在 0 0 0 5～ 1 0 0 0mg/mL范围内 ,含量均与其峰面积之间呈现良好的线性关系。该法灵敏度高 ,成本低 。

A capillary electrophoretic method for determinating main components in isomaltooligosaccharide formulation was describled The influences of various separartion conditions including buffer conceration,pH,voltage and detection wavelength were investigated By using an uncoated silica capillary (75?μm i d ,60cm of total length,and 50?cm of efficient length) and 75?mmol/L borax (pH10 5) as carrier electrolyte,the sugar derivatized with α naphymethamine,including isomaltose,panose,isomaltotriose,were sufficiently separated at an applied voltage of 15?kV and a UV detection wavelength of 214?nm The results indicate that the relative standard deviations for migration times and peaks area w2澹颍? all less than 0 5% and 2 0% respectively Quantification by the peak area method allowed reproducible analysis at concentration ranges from 0 005 to 1 000?mg/mL This method has the characteristics of sensitive,reproducibility and low cost. It should be useful for the determination of main components of isomaltooligosaccharide formulation