超滤法分离纯化沙丁鱼肽

Study on UF for the Separation and Purification of Sardine Peptide

用板式超滤装置以全回流方式对沙丁鱼粗肽溶液进行处理 ,通过测定不同水解度的沙丁鱼粗肽溶液在超滤过程中组分的透过速率、截留液和透过液中肽的含量 ,及SDS 聚丙烯酰胺电泳检测组分分子质量的分布 ,对超滤膜的选择性及分离效果进行了评价和分析。试验结果表明 ,超滤膜对沙丁鱼粗肽溶液中不同分子质量的组分具有分离纯化作用 ,超滤技术可以作为一种初步检测沙丁鱼粗肽溶液组分分子质量分布的手段。

Sardine peptide was separated and purified by ultrafiltration membrane.By measuring the change of permeate flux, the content of peptides in the sardine peptide solution during UF process, and molecular weight distribution of the peptides using SDS-PAGE electrophoresis, the selectivity and the separation effect were analyzed and evaluated.It was showed that the component with different molecular weight can be separated and purified by filtrating membrane, and UF can be used as a preliminary measuring method to analyze the *#molecular weight of the peptide solution.