三个HLA-B分子影响血清阴性脊柱关节病外周血NK细胞毒活性的研究

Influences of three different HLA-B antigens expression on K562 cells on cytotoxicity of peripheral natural killer cells from seronegative spondyloarthropathies patients

目的探讨HLA-B27、B39和B51抗原分别在K562细胞表达后,对血清阴性脊柱关节病(SpA)外周血NK细胞毒活性的影响。方法采用乳酸脱氢酶(LDH)释放法,分别测定9例SpA病人和9名正常人外周血NK细胞对表达HLA-B27、B39和B51抗原的K562细胞及转染空pcDNA3质粒的K562细胞的杀伤效应。结果SpA病例组和正常对照组均显示B27和B51抗原表达于K562细胞后,能阻断NK细胞毒活性,而B39不能阻断NK细胞毒性;当比较病例组和正常组之间NK细胞分别对K562,K562-B27、B39和B51细胞的杀伤效应时,显示两组外周血NK细胞对K562细胞、转染B27、B39和B51分子的K562细胞的毒性差异均无显著性(P>0.05)。但是,SpA病例组中NK细胞对HLA-B27转染的K562细胞杀伤效应与正常组相比,虽然差异未达到显著水平,但显示了一定程度的偏低(P<0.1)。结论K562细胞表达HLA-Bw4分子后,明显抑制NK细胞的杀伤效应,而表达HLA-Bw6分子对NK细胞杀伤功能无明显影响;而且提示B27阳性的SpA患者体内,外周血NK细胞毒活性偏低。

Objective To investigate the influences of three different HLA-B antigens expression on K562 cells on cytotoxicity mediated by NK cells from PBMC of SpA patients. Methods NK cells from 9 samples of SpA patients and 9 samples of normal control were randomly selected to detect their cytotoxicity on K562 cells which were transfected with HLA-B27,B39,B51 and null pcDNA3 plasmid respectively by the LDH method. Results In contrast with null K562-pcDNA3,K562-B27 and K562-B51 could inhibit NK cytotoxicity significantly,whereas K562-B39 had no inhibition neither in patient group nor in the normal control group.In general,there was no significant difference of cytotoxicity on K562-pcDNA3,K562-HLA-B39,K562-HLA-B27 and K562-HLA-B51 between SpA patients and normal controls.Although the difference didn't reach the significant level,NK cells from SpA patients showed decreased cytotoxicity on HLA-B27 transfected K562 cells (P<0.1). Conclusion HLA-Bw4 and HLA-Bw6 molecules have different influences on NK cell cytotoxicity,and peripheral NK cells from HLA-B27 positive SpA patients may have relatively lower cytotoxicity.