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绿豆Ty1／Copia类反转录转座子逆转录酶序列的克隆和分析 被引量：1

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摘要 利用Ty1／copia类反转录转座子的保守位点设计简并引物，从绿豆(Vigna radiata(L.)Wilczek)基因组中扩增得到了反转录转座子的逆转录酶序列。对扩增得到的约270 bp的片段进行分离和克隆，并随机挑选了40个克隆进行测序，结果得到了36个单独的核酸序列，其中18个含有移码突变或终止子。根据序列比对，这些克隆可分为9组以及单个的9种。这40个克隆中，核酸序列相似性从8％到100％，显示出其核酸序列的高度异质性。将这些克隆的核酸序列与来自其他种植物的相应序列进行谱系分析，发现有些克隆与来自其他种植物的相应序列的亲缘关系比这些克隆之间更为接近。斑点杂交显示Ty1／copia反转录转座子约占绿豆基因组的9．3％。
作者 肖卫民 坂本亘 苏都莫日根
机构地区 北京大学生命科学学院 ResearchInstituteforBioresources
出处 《Acta Botanica Sinica》 CSCD 2004年第5期582-587,共6页 Acta Botanica Sinica（植物学报：英文版）
关键词 绿豆 DOP-PCR COPIA 反转录转座子 逆转录酶
分类号 Q943 [生物学—植物学]
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参考文献34

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同被引文献18

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Acta Botanica Sinica
2004年 第5期

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