急性缺氧和急性低糖对脑片tau蛋白磷酸化的影响

Effect of Acute Hypoxia on tau Phosphorylation in Rat Brain Slices

为探讨急性缺氧对tau蛋白磷酸化的影响 ,将Wistar大鼠脑片进行不同时间的缺氧培养后 ,对tau蛋白的磷酸化状态及相关磷酸酯酶的活性和表达进行检测 .结果显示 ,急性缺氧使tau蛋白多个丝氨酸位点磷酸化水平下降 ,蛋白磷酸酯酶 2A (PP 2A)的活性升高 ,其催化亚单位表达上调 ,而蛋白磷酸酯酶 1(PP 1)的活性及催化亚单位表达均无明显改变 .该研究结果表明 :急性缺氧可能通过蛋白磷酸酯酶

To explore the effect of acute hypoxia on tau phosphorylation, rat brain slices were incubated at 37degreesC for 30 min or 120 min with or without oxygen supply. Then tau phosphorylation was detected by Western blots. The results showed that significant dephosphorylation of tau at Ser-396/Ser-404, Ser-422 and Ser-199/Ser-202 was induced by acute hypoxia. The activity of protein phosphatase-2A ( PP-2A) and the expression of PP-2A catalytic subunit were simultaneously increased. The results suggest that acute hypoxia induces dephosphorylation of tau and the up-regulation of PP-2A may be at least one of the underlying mechanisms.