基于图像信息处理的蒜核仁内DNA原位排布及其结构模型的研究

Studies on the Location of Nucleolar DNA In situ in Allium sativum Cells and Its Structural Model by Image Processing

真核细胞核仁内的DNA超微结构对于阐述rRNA转录位点有着重要的意义 .虽然已经有许多研究者对此提出了不同的观点 ,但是都是基于直接实验观察的 .应用电子显微镜技术和改进的NAMA UrDNA特异染色方法 ,获得蒜核仁超微结构的图像 ,提出一种新技术定量分析核仁中DNA原位位置 .为此 ,建立多尺度形态梯度算子方法 ,编制HEREN .CELL自动分析软件 ,从而抽取出核仁超微结构的精细轮廓 ,显示出核仁DNA的原位位置 .同时 ,提出了DNA纤丝以“花瓣包迹”模式向中心外方向放射的结构模型 .

Ultrastructure of nucleolar DNA is most important to elucidate transcription site of rRNA. Many judgments were made in this respect but all of them were based on direct experimental observations. A new technology for the quantitative analysis of the location of nucleolar DNA in situ from EM data of the ultrastructure of the nucleolus in allium sativum cells and from the modified NAMA-Ur DNA specific staining, method was developed. For this purpose, a new methodology of multi-scale morphological gradient operator and automatic analysis software named 'HEREN. CELL' were created and adopted. By using this method, superfine contour of the nucleolar ultrastruture was obtained and showed the location and location of nucleolar DNA in situ. From the results, a 'petaline envelope' model was proposed, which indicated that nucleolar DNA fibrils radiate from center to periphery of the nucleolus. The study will contribute to the development of the EM image processing technology and the study of nucleolar ultrastructure of the nucleolus.