摘要
为了构建白藜芦醇合成酶 RS 基因的克隆载体,用PCR技术从葡萄叶片总DNA中扩增出RS基因全长,然后将其重组到克隆载体中.通过酶切对重组质粒进行了鉴定和测序,结果表明,RS基因已经正确克隆至pUC19中,将重组质粒转入大肠杆菌里,使RS基因能够在大肠杆菌里保存并且大量扩增,为RS基因构建表达载体表达白藜芦醇合成酶奠定了基础.
Resveratrol synthase gene was amplified by PCR .Then it was inserted into pUC19 .The recombinant vector was verified with restriction analysis.The result showed that the RS gene was cloned correctly into pUC19 .It's concluded that the vector was constructed successfully.
出处
《西北植物学报》
CAS
CSCD
2004年第6期1007-1011,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省自然科学基金资助项目 (99JK122 )
