中国人Canstatin基因的克隆

Molecular clone and homologous sequence analysis of canstatin cDNA derived from chinese hepatocytes

目的 Canstatin是新近发现的血管生成抑制剂 ,具有很强的抑制肿瘤血管生成的功能 ,本研究拟克隆和鉴定中国人的Canstatin基因 ,为进一步研究Canstatin基因的功能奠定基础。方法 用RT PCT法从中国人肝组织中获得Canstatin的cDNA ,克隆到pCMV Script载体中测序 ,并在GenBank中进行同源性分析。结果 成功克隆了Canstatin基因cDNA编码序列并获得测序证实。结论 中国人Canstatin基因编码序列 ,经同源性分析与国外文献报道序列一致 ,初步证明该基因可能属于人类基因中序列保守的基因。

Objective Canstatin is a new endogenous angiogensis inhibitor.To study its further function,the canstatin cDNA from Chinese fetus hepatocytes and para hepatocarcinomatous normal tissue was cloned and sequenced.Methods The Canstatin cDNA coding sequences were acquired by RT PCR metods from Chinese fetus hepatocytes and para hepatocarcinomatous normal tissue were cloned into pCMV Script vector and sequenced.Result The canstatin cDNA coding sequence was successfully cloned and sequenced.Conclusion The homologous sequence analysis showed that the coding sequence of Chinese canstatin had no difference from that of foreigner reported.Canstatin was proved probably to be one of the conservative gene in human genome.