人大肠良恶性病变组织中端粒酶活性的表达

Expression of telomerase activity in human benign colorectal lesions and colorectal cancers

目的 研究端粒酶活性在大肠癌、癌旁组织、正常大肠黏膜及大肠良性病变组织中的表达情况 ,探讨其作为大肠癌生物学标志物的可能性。方法 PCR TRAP ELISA法检测 30例大肠癌、癌旁组织及正常黏膜和 2 0例大肠腺瘤性息肉组织中端粒酶活性水平。结果 (1 ) 30例大肠癌组织、癌旁组织、正常大肠组织及 2 0例大肠腺瘤性息肉组织中端粒酶活性分别为83 33% ,1 3 33% ,3 33%和 1 0 0 0 % ,癌组织中端粒酶活性明显高于其他组织 (P <0 0 5 ) ;(2 )伴淋巴结转移大肠癌端粒酶阳性表达率为 80 % (8/ 1 0 ) ,无淋巴结转移者为 0 % (0 / 2 0 ) ,二者比较有非常显著性差异 (P <0 0 0 1 ) ;(3)大肠癌组织端粒酶活性随组织学分级、临床分期而呈逐渐增高趋势 ,但统计学处理差异无显著性 (P >0 0 5 )。结论 端粒酶活性的检测可作为大肠癌诊断的指标之一 ,对判断大肠癌的预后具有重要参考价值。

Objective To investigate the telomerase activity in normal sucoma,benign and malignant lesions of colorect,and to explore the possibility of telomerase as a tumor biological marker Methods Telomerase activity was measured in 30 colorectal carcinoma,paracancerous tissues,normal sucoma and 20 adenomatous polyp by PCR TRAP ELISA assay Results (1)The expression of telomerase activity was 83 33%,13 33%,3 33%,10 00% in colorectal carcinoma,paracancerous tissues,normal sucoma and adenomatous polyp tissues ,respectively The activity of telomerase in colorectal cancer tissues was significantly higher than that in others tissues(P<0 05) (2)Telomerase activity was detected in 8 of 10(80%) colorectal cancerous specimens with lymphatic matastasis,and none of 20 samples without lymphatic matastasis(P<0 001).(3)Telomerase activity was independent of histological classification and clinical stage(P>0 05).Conclusions The activity of telomerase might be a diagnostic marker of colorectal carcinoma,and might play an important role in prognosis of colorectal carcinoma.