纳洛酮对心肺复苏后犬肺组织炎症因子水平及其mRNA表达的影响

Influences of naloxone on the concentration and expression of lung cytokine after cardiopulmonary resuscitation in dogs

目的 检测犬心肺复苏后 6h ,肺组织TNF α、IL 6浓度和肺中TNF αmRNA、IL 6mRNA、IL 10 mRNA的表达变化 ,以探讨纳洛酮对肺组织中TNF α、IL 6、IL 10含量的影响。方法 18只健康杂种犬 ,随机分成 3组 ,空白组 (n =6 ) :不诱发室颤 ,6h后取肺组织 ;对照组 (n =6 ) :心跳骤停后予常规心肺复苏术 ,实验组 (n =6 ) :心跳骤停后予常规心肺复苏术 +纳洛酮。于复苏后 6h取肺组织行TNF α、IL 6浓度和肺TNF α;mRNA、IL 6mRNA、IL 10mRNA的表达的测定以及行肺形态学检查。结果 实验组TNF α浓度明显低于对照组 (P <0 0 1) ,实验组、对照组、空白组IL 6浓度无差别 (P >0 0 5 )。实验组IL 10mRNA的表达明显高于对照组 (P <0 0 5 ) ,实验组和对照组的TNF αmRNA、IL 6mRNA的表达无差别 (P >0 0 5 )。实验组肺组织的病理损害低于对照组。结论 纳洛酮可减轻心跳骤停后肺组织的TNF α的生成 ,使IL 10mRNA的表达增加 ,从而减轻心肺复苏后肺的再灌流损伤。

Objective To observe influence of concentration of lung TNF-α, IL-6 and the expression of lung TNF-α,IL-6 and IL-10 6 hour after cardiopulmonary resuscitation(CPR) in dogs.Methods Group 1(n=6)lung tissue was taken without cardiac arrest after 6 hour. Group 2 (n=6) was performed standard CPR after cardiac arrest in dogs. Group3 were group 2+naloxone. Lung tissue was taken out 6 hours after CPR. The concentrations of lung TNF-α,IL-6 and the expressions of lung TNF-α, IL-6 and IL-10 were measured and lung tissue was also used for histological examination.Results The concentration of lung TNF-α in group 2 was significantly higher than in group 3(P<0.01)and group 1(P<0.01).The mRNA expression of lung IL-10 in group 3 was significantly highter than in group 2(P<0.05) and in group I(P<0.01).The mRNA expressions of lung TNF-α,IL-6 in three groups were not statistically different.Pathological lung injury in group 2 was significantly lower than in group 3. Conclusion Natoxone can attenuate ischemia reperfusion lung injury after CPR through reducing TNF-α and increasing the mRNA expression of lung IL-10.