大鼠全脑缺血再灌注时海马CA_1区IL-10变化的实验研究

The significance of expression of IL-10 in rats after global cerebral ischemia-reperfusion

目的 观测全脑缺血再灌注时海马CA1区白细胞介素 - 10mRNA (IL - 10mRNA)、肿瘤坏死因子 -αmRNA (TNF -αmRNA)、白细胞介素 - 1βmRNA (IL - 1βmRNA)表达的变化 ,探讨全脑缺血再灌注时IL - 10表达的作用。方法 按照Pullsinelli法建立大鼠全脑缺血再灌注损伤模型 ,分别于缺血再灌注 2、 6、 12、 2 4、 4 8h取脑海马CA1区组织切片 ,用原位杂交法和图像分析检测IL - 10mRNA、TNF -αmRNA、IL - 1βmRNA的吸光度值。结果 脑缺血再灌注各时间点海马CA1区TNF -αmRNA、IL - 1βmRNA的吸光度值均显著增加 (P <0 0 1) ,4 8hIL - 10mRNA显著表达 (P <0 0 1) ;2 4～ 4 8hIL - 10mRNA的吸光度值与TNF -αmRNA、IL - 1βmRNA的吸光度值均呈显著负相关(r值分别为 - 0 70 6、 - 0 84 2 ,P值 <0 0 5 )。结论 大鼠全脑缺血再灌注 4 8hIL - 10显著表达 ,对TNF -α、IL - 1β具有显著的抑制作用。

Objective To explore the effect of IL-10,we detected IL-10mRNA and TNF-α mRNA and IL-1βmRNA In rat brain hippocampus CA 1 regions after global cerebral Ischemia-reperfusion.Methods 50 Wistar rats were divided into Sham operation(S)group and ischemia-reperfusion(IR)group.The global cerebral Ischemia-reperfusion animal models were formed by Pullsinelli′s method.The hippocampus CA 1 tissues at 2h,6h,12h,24h,and 48h were taken out respectively after sham operation and global cerebral Ischemia-reperfusion,then the expressions of IL-10mRNA and TNF-α mRNA and IL-1βmRNA were examined by In situ hybridization.Results The changes of all observed targets were as the following:(1) At 48h after global cerebral ischemia-reperfusion,while the expression of IL-10mRNA was Increased significantly (P<0 01),that of TNF-αmRNA and IL-1βmRNA were decreased rapidly.(2) The expressions of TNF-αmRNA and IL-1βmRNA at every time points were Increased significantly (P<0 01),respectively.3 There was negative correlation between IL-10mRNA and TNF-αmRNA and IL-1βmRNA(r=-0706,-0842,P<0 05 or <0 01)respectively.Conclusion The expression of IL-10mRNA,at 48h after global cerebral Ischemia-reperfusion,could reduce obviously expressions of TNF-αmRNA,IL-1βmRNA.