Thapsigargin对乳腺癌MCF-7细胞凋亡的诱导作用研究

Research on the Induction of Apoptosis in MCF-7 Cells Enhanced by Thapsigargin

目的 探讨Thapsigargin对人乳腺癌MCF 7细胞株雌激素受体阳性的凋亡诱导作用。方法 在体外培养条件下 ,应用Thapsigargin与 5 FU处理MCF 7细胞株 ,采用流式细胞仪检测其细胞凋亡率和周期分布 ;MTT比色法分析其细胞生长抑制率 ;透射电镜观察细胞超微结构。结果 Thapsigargin可使 5 FU诱导的MCF 7细胞凋亡率和生长抑制率明显增加 ,并改变细胞周期的分布。电镜下可见MCF 7细胞凋亡小体明显增加。结论 Thapsigargin具有明显加强 5 FU对人乳腺癌MCF 7细胞株的凋亡诱导作用 ,值得进一步研究。

Objective To study the apoptotic induction effect of Thapsigargin on estrogen receptor positive human breast cancer cell lines MCF 7. Methods Cells were treated with Thapsigargin and 5 FU in vitro . The rate of cell apoptosis and distribution of cell cycle were detected on flow cytometry. The cell viability was measured by MTT assay and ultrastructural changes in apoptotic cells were confirmed by transmission electron microscopy.Results Thapsigargin could increase the rates both of cell apoptosis and growth supression of MCF 7 cells induced by 5 FU and alter the distribution of cell cycle. Under electron microscope, apoptotic bodies in MCF 7 cells considerably increased.Conclusion Thapsigargin apparently enhances the effect of apoptotic induction of 5 FU on MCF 7 cells, it is worthy of being further studied.