LBP多抗对内毒素急性肺损伤大鼠肺泡巨噬细胞IL-10、IL-18基因表达的影响

Effects of polyclonal antibody to lipopolysaccharide binding protein on IL-10 and IL-18 mRNA expressions of alveolar macrophages in lipopolysaccharide-induced acute lung injury in rats

目的 探讨脂多糖结合蛋白 (lipopolysaccharidebindingprotein ,LBP)多抗对内毒素急性肺损伤大鼠肺泡巨噬细胞IL 10、IL 18基因表达的影响 ,为LBP多抗治疗急性肺损伤提供实验依据。方法 Wistar雄性大鼠 40只 ,随机分段等分为 5组 ,A组为正常对照组 ;B组为LPS处理组 ;C组为LPS注射前 3 0min加LBP多抗预处理组 ;D组为LPS和LBP多抗同时处理组 ;E组为LPS注射后 3 0min加LBP多抗处理组。分离培养各组大鼠肺泡巨噬细胞 ,用RT PCR方法测定其IL 10、IL 18mRNA表达。结果 LPS显著增加IL 10、IL 18mRNA表达 (B组 ) ;LBP多抗能显著降低二者的表达 ,尤以预处理组(C)更明显。结论 LBP多抗能显著降低内毒素急性肺损伤炎症因子的IL 10、IL

Objective To explore the effects of polyclonal antibody to lipopolysaccharide binding protein (pLBPab) on IL 10 and IL 18 mRNA expressions of alveolar macrophages in lipopolysaccharide LPS induced acute lung injury in rats. Methods A total of 40 male Wistar rats were randomly divided into 5 groups: normal control (A), LPS treated group (B), group of pLBPab preconditioning at 30 min before injection of LPS (C), group of treatment with LPS and pLBPab (D), and group of pLBPab preconditioning at 30 min after injection of LPS (E). mRNA expressions of IL 10 and IL 18 in the alveolar macrophages in each group were measured by semi quantitative reverse transcription polymerase chain reaction (RT PCR). Results The IL 10 and IL 18 mRNA expressions were highly increased respectively in the alveolar macrophage (AMφ) stimulated with single LPS, but they were significantly decreased in the AMφ after stimulation with LPS + pLBPab, particularly stimulation with pLBPab 30 min before LPS injection. Conclusion pLBPab can decrease the mRNA expressions such as IL 10 and IL 18 by alveolar macrophages in acute lung injury in rats induced by LPS, and LBP antibody could be used to cure some diseases such as SIRS, acute lung injury, ARDS, and septic syndrome.