干扰素α对Raji细胞的生长抑制作用及其作用机制(英文)

Anti-proliferation effect of interferon-α on Raji cells and its mechanism

目的 :探讨干扰素α对白血病Raji细胞生长的抑制作用及其作用机制。方法 :以不同浓度的干扰素α作用于体外培养的Raji细胞 ,应用MTT法检测细胞生长抑制率 ,流式细胞仪检测细胞凋亡率 ,Hoechst 332 5 8荧光染色法观察细胞凋亡 ,TRAP PCR ELISA法检测细胞凋亡前后的端粒酶活性。结果 :5× 10 3 U·ml-1以上的干扰素α可显著降低Raji细胞端粒酶活性 ,抑制细胞的生长及诱导细胞发生凋亡 ,并呈现出明显的量 效与时 效关系。药物 (10×10 3 U·ml-1)作用 4 8～ 6 0h在Hoechst染色图片上可见核浓缩及核碎裂等典型的凋亡改变。结论 :干扰素α能抑制Raji细胞的生长并诱导细胞发生调亡 ,降低细胞端粒酶活性可能是其重要作用机制之一 ;

AIM: To investigate the anti proliferation effect of interferon α(IFN α) on leukemic Raji cells and its mechanism. METHODS: Raji cells were given different concentrations of INF α. The inhibitory rate of the cells was measured by MTT assay, apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by Hoechst 33258 fluorescence staining, and the activity of telomerase was detected by PCR ELISA before and after apoptosis occurred. RESULTS: IFN α (over 5× 10 3 U·ml -1 ) could cause apoptosis significantly, decrease the telomerase activity, and inhibit the growth of Raji cells in time and dose dependent manner. Marked morphological changes of cell apoptosis including chromosome condensation and nuclear fragmentation were observed very clearly by Hoechst 33258 fluorescence staining especially after the cells treated with IFN α for 48-60 h. CONCLUSION: IFN α has apparent anti proliferation and apoptotic effects on Raji cells in vitro, and one of the most important mechanisms may be decrease of the telomerase activity of Raji cells. These results will provide strong laboratory evidence of IFN α in the clinical treatment of lymphoma cell leukemia.