用沙门氏菌复制兔“热毒血瘀证”病理模型的研究

The Reconstruction of Rabbit Model with Blood Stasis Syndrome Due to Febrile-Toxication by S. Pullorum

为了探讨温病血瘀的形成机理,采用肌注沙门氏菌的方法,复制了兔“热毒血瘀证”模型,并对模型兔的血液流变性、血液凝固性等方面进行了研究。结果表明:全血粘度在切速为230s^(-1),115s^(-1)和23s^(-1)下分别为0.004684±0.000455pa·s,0.005093±0.000515pa·s和0.00678±0.000789pa·s;全血还原粘度为0.0147±0.00076 pa·s/％;血浆粘度为0.008185±0.000334pa·s;血沉为16.9±3.0mm/h;血沉k值为35.7±8.30,均显著高于正常对照组(p<0.01)。红细胞压积为31.9±2.6％;红细胞电泳速率为3.5±0.31μm/s,均显著低于正常对照组(p<0.01),血小板聚集性为58.21±4.05％;血小板免疫粘附功能为1.09±0.33;血浆纤维蛋白原含量为1.47±0.28g％;优球蛋白溶解时间为37.30±11.14h,均显著增加(p<0.001),血小板数为40.06±3.24万/mm^3;血小板电泳速率为4.71±0.34μm/s均显著降低(p<0.001)体外血栓形成的长度为57.22±9.07mm;湿重215.93±10.85mg;干重61.02±9.48mg,均显著高于正常对照组(p<0.01)。临床呈现出高热、呼吸心率加速、体表血管充血或有瘀血斑、口色瘀紫色及内脏组织器官的病理损伤。实验结果认为以沙门氏茵接种于兔所复制的病理模型符合中医、中兽医有关热毒和血瘀的理论,当属“热毒血瘀证”模型。

In order to study the mechanism of blood stasis due to accute febrile disease, we established a rabbit model of blood stasis due to intense evil heat by the injection of S.pullorum and studied lt's hemorrheology, blood coagulation and fibrinolytic function. The results showed that in the treatment group the whole blood viscosity under 230s^(-1), 115s^(-1) and 23s^(-1) were 0.004684±0.000455pa·s, 0.005093±0.000515pa·s and 0.00678±0.000789pa.s, respectively. the reducing viscosity of whole blood was 0.0147±0.00076 pa·s/%, the blood plasma viscosity was 0.002185±0.000334 pa·s, the erythrocyte sedimentation was 16.90±3.0mm/h and the k value of erythrocyte sedimentation was 35.70±8.30. They were all higher than those in control group (P<0.01). The hematocrit was 31.90±2.60% and the RBC electrophoresis rate was 3.50±0.31μm/s in treatment group, and they were lower than those in control group(P<0.01). The platelet saggregation was 58.21±4.05%, the platelet immune adherence was 1.09±0.33, the fibrinogen content in plasma was 1.47±0.28 g% and the euglobulinlysis time was 37.30±11.14h in treatment group, and they were increased(P<0.001) as compared with those in control group. The platelet count (40.06±3.24万/mm^3) and platelet electrophoresis rate(4.71±0.34μm/s) in treatment group were decreased(P<0.001) after the injection of s.pullorum, the length(57.22±9.07 mm), Wwet(215.93±10.85mg) and Wdry(61.02±9.48mg) of extracorporeal thrombosis in the treatment group were higher than in the control group. The rabbits in the treatment grorop appeared high fever, acceleration of respiratiou and heart rate, hyperemia of the peripheral blood vessel or formed ecchymosis. purple tongue and pathological changes of the viscera. On the basis of these results, the authors conclude that the rabbit model of pathology caused by the injection of S. Pullorum accord with the theory and character of traditional chinese medicine and veterinary medicine about Febrile-Toxication and blood stasis and belong to the model with blood stasis syndrome caused by Febrile-Toxication.