获得性大疱性表皮松解症的免疫病理诊断

IMMUNOPATHOLOGICAL DIAGNOSIS ON EPIDERMOLYSIS BULLOSA ACQUISITA

采用皮肤活检直接免疫荧光法(DIF)和血清间接免疫荧光法(IIF)确诊两例获得性大疱性表皮松解症(EBA).两例皮疹和其中一例另四处正常皮肤的 DIF均显示皮肤基底膜带(EMZ)有线状 IgG 和 C_3沉积.用1mol/L 氯化钠溶液分离皮肤作抗原性底物,IIF 显示线状 IgG 荧光带沉积在真皮一侧,滴度为1:20.

Both direct immunofluorescence staining(DIF)of skin biopsy and indirect immunofluorescence test (IIF)of serum offered valuable adjuncts to clinical and histological diagnosis in two patients with epidermolysis bullosa acquisita(EBA).DIF of the lesions of two cases and four different sites of skin of one case revealed linear labelling of basement membrane zone(BMZ)with IgG and C_3.IgG IIF of two cases were performed on the substrates using 1 mol/L NaCl to separate human skin through the lumina lucida.The results of IIF of EBA demonstrated dermal pattern of linear IgG immunofluorescence zone,and the titers of sera were 1:20.