碱性大环内酯类抗生素的微生物受体竞争结合分析

Microbial receptor competitive binding assay of basic macrolide antibiotics

目的建立不同基质中大环内酯含量的分析方法。方法血清、尿液和组织样品经MSU缓冲液稀释萃取。谷物和预混饲料经甲醇-磷酸盐溶液提取后进行微生物受体竞争性结合反应分析,缓冲液M8可消除甲醇对结合反应的干扰。结果该法族特异性强,灵敏度和精密度高,血清、尿液、组织和谷物预混饲料的限量筛选浓度为200、200、100和1 200 ng/g (ng/ml),相对标准偏差<8%,可对大环内酯类药物进行总量测定。结论该方法准确、快速,能实现不同基质样品的高效定性和半定量筛选。

Objective To establish a biological method for determining the macrolide content in various matrices. Method Human serum, urine and tissue homogenate samples were diluted or extracted with MSU buffer, and the specimens of grain and premixed feed extracted with methanol-H3PO4 buffer, before microbial receptor competitive binding assay was carried out on these various specimens, with the elimination of interference from methanol with the M8 buffer. Results The method was sensitive, class-specific and precise, and the recommended screening concentrations for specimens of the serum, urine, tissue and grains were 200, 200, 100 and 1 200 ng/g (ng/ml), respectively, with a relative standard deviation less than 8%. Conclusion Microbial receptor competitive binding assay is accurate and rapid for efficient qualitative and quantitative assay of the total macrolide content in various matrices.