一氧化氮阳性神经元在缺氧预适应中的变化

Changes of NOS neuron in proconditioning to hypoxia

目的 :探讨缺氧预适应形成机理。方法 :采用组化法测定小鼠脑内一氧化氮合酶 (NOS)神经元在缺氧 (1次缺氧 ,4次缺氧 )预适应中的变化。结果 :1次缺氧组较正常对照组皮层的 NOS阳性神经元的数目、强弱、细胞的形状、突起的数目及突起的长短未见明显变化 ,但 1次缺氧组 NOS神经元突起明显变粗 ,4次缺氧组与正常对照组比较无明显变化。正常对照组海马 NOS阳性神经元数目较少 ,且呈弱阳性 ,1次缺氧组海马 NOS阳性神经元数目增加 ,多呈强阳性 ,4次缺氧组海马 NOS阳性神经元较 1次缺氧组数目变少 ,颜色变浅。结论 :脑内

Objective To explore the mechanism of proconditioning to hypoxia. Methods The changes of NOS neuron of mouse brain in proconditioning to hypoxia were detected by histochemistry method.Results Compared with control mice, the number, colour, shape, dendrites and axons number and length of NOS positive neurons in cerebral cortex was not changed after mice were exposed to hypoxia for one time, but the dendrites and axons of NOS neuron became thicker. Compared with control mice, NOS neurons in cerebral cortex was not changed after mice were exposed to hypoxia for four times. There were little NOS neurons in hippocampus of control mice and NOS neurons were light positive. Compared with control mice, the number of NOS positive neurons in hippocampus increased and NOS neuron became strong positive after mice were exposed to hypoxia for one time. Compared with mice exposed to hypoxia for one time, the number of NOS positive neurons in hippocampus decreased and their colour became light after mice were exposed to hypoxia for four times. Conclusion The changes of NOS neurons can enhance generation of proconditioning to hypoxia.