胎鼠皮肤间充质干细胞的体外培养及成骨分化研究

Study on differentiation of fetus mice skin MSCs into osteoblasts cultured in vitro

目的 :分离、纯化胎鼠皮肤间充质干细胞 (MSCs) ,在体外培养、扩增并促其成骨分化 ,为骨组织工程学研究与骨质疏松症的细胞学治疗提供一种可能。方法 :体外培养胎鼠皮肤 MSCs,并在培养液中加入成骨诱导剂诱导培养 ,观察其成骨分化潜能。结果 :胎鼠皮肤 MSCs传代培养第 1 5代仍具有增殖能力 ,说明该细胞为干细胞源性 ;贴壁细胞在成骨诱导剂作用下 ,碱性磷酸酶 (AL P)水平升高 ,AL P钙钴染色呈阳性反应 ,钙结节茜素红染色反应阳性。结论 :胎鼠皮肤 MSCs具有很强的自我增殖能力 。

Objective The characteristics of the proliferation and differentiation of fetus mice skin MSCs were observed in order to provide an ideal method for bone tissue engineering science and treatment for osteoporosis. Methods Fetus mice skin MSCs were purified with the method of cell culture in vitro and potential of differentiation were observed in culture fluid with the osteogenic inducer.Results The repeated adherent passage cultured cells that were originated from the fetus mice skin cells still had a strong proliferation capacity after 15 generation′s passage purification culture. It was clarified that the cultured cells were originated from stem cells. The adherent cultured cells increased the activity of ALP, ALP staining reaction and calcium nodus alizarin red staining reaction were positive with the osteogenic inducer. Conclusion The skin MSCs existing in the fetus mice have the potential of self proliferation and can be differentiated into osteoblasts with the osteogenic inducer.