广东省2002年急性弛缓性麻痹病例病原流行病学分析

Analysis on Pathogenic Epidemiology of Acute Flaccid Paralysis of Guangdong Province in 2002

目的对广东省2002年急性弛缓性麻痹(AFP)病例进行病原流行病学分析。方法收集2002年报告的AFP病例粪便标本292份,进行病毒分离与鉴定,对2002年AFP病例的免疫史、性别、年龄、60d随访结果与病原学结果之间的关系以及三种细胞对AFP病例粪便标本的病毒分离结果进行分析。结果分离到脊髓灰质炎病毒(PV)22株,阳性率为7.53%,均为疫苗株,其中Ⅱ型病毒(PVII)12例,为优势株;分离到非脊灰肠道病毒(NPEV)15株,阳性率为5.1%,低于1994～2001年。脊灰减毒口服活疫苗(OPV)“零”剂次免疫的AFP病例的PV分离率依次高于1～2次和完成3次以上(≥3)全程免疫者,达30.0%;15例无明确诊断的残留麻痹病例的PV分离率远高于其他病例,为33.3%;应用Hep-2细胞分离阳性率高于RDa与L20B细胞,分别为19.2%、11.3%和7.5%,三种细胞分离率比二种细胞分离率提高11.7%。结论2002年广东省没有发现由脊灰野生型病毒引起的病例。

Objective To analyze the pathogenic epidemiology of AFP(acute flaccid paralysis ) of Guangdong in 2002. Methods The feces specimens of 292 AFP cases were collected,and conducted for virus isolation and identification. The correlation of the immunization history, sex, age and 60 days follow up result of AFP cases were analyzed. Virus isolation and identification of AFP cases feces specimen were conducted with RDa、L20B and Hap 2 cells. Results 22 PV(poliovirus)strains were isolated with 7.53% of the positive rate . All of them were vaccine strains, of which 12 strains were PVⅡ(predominant strains). 15 NPEV were isolated with a positive rate of 5.1%. It is lower than the level from 1994 to 2001. The PV positive rate of AFP cases with zero dose OPV immunization was 30.0% and which was higher than that of the cases with 1～2 doses and 3 doses immunization. The PV positive rate was 33.3% in the 15 cases of indeterminable residual paralysis, which was higher than that in other cases. The isolation positive rate using Hap 2 cells was higher than using other cells. Conclusion No polio case caused by wild poliovirus was found of Guangdong province in 2002.