益气、活血、益气活血法对气虚血瘀证脑缺血再灌注模型鼠血液流变性的影响

Effect of Therapy of Qi-supplementing,Blood-activating and Qi-supplementing & Blood-activating on Hemorrheology in Rats with Qi Deficiency & Blood Stasis Syndrome Following Cerebral Ischemia-reperfusion

目的 :观察益气、活血、益气活血法对气虚血瘀证局灶性脑缺血再灌注模型鼠血液流变性的影响。方法 :采用饥饿、劳累、高脂饮食等多因素复合制备气虚血瘀证模型 ,然后用线栓法阻断大脑中动脉复制局灶性脑缺血再灌注模型。实验动物随机分为假手术组、模型组、益气组、活血组、益气活血组。给药后 ,分别在脑缺血再灌注 1 ,3,7d检测血液流变学指标。结果 :与模型组比较 ,各治法组均有显著改善血液流变性的作用 (P <0 .0 5或P <0 .0 1 ) ,各治法组间比较 ,差异没有显著性 (P >0 .0 5 )。结论 :益气、活血、益气活血法改善脑缺血模型鼠的血液流变性可能是其抗脑缺血再灌注损伤的作用机制之一。

Objective: To study the effect of therapy of Qi supplementing,blood activating,Qi supplementing & blood activating on hemorrheology in rats with Qi deficiency & blood stasis syndrome following cerebral ischemia reperfusion. Method: The Qi deficiency & blood stasis model was made by means of combined factors of hunger, tiredness and high fat diet , furthermore local cerebral ischemia reperfusion model was completed by blocking middle cerebral artery with thread occluding ways. The experimental animals were divided into pseudo surgery group,model group, Qi supplementing group, blood activating group and Qi supplementing & Blood activating group. Hemorrheology indexes were measured in 1th, 3rd, 7th day after reperfusion. Result:The Qi supplementing,blood activating,Qi supplementing & blood activating therapy may significantly improve hemorrheology indexes(P<0.05 or P<0.01). There is no difference among three treatment groups(P>0.05).Conclusion:The protective effects of Qi supplementing,blood activating,Qi supplementing 8x blood activating therapy against cerebral ishemia reperfusion injury might associate with improving hemorrheology indexes.