PTEN、VEGF在肝细胞癌中的表达及临床意义

Expression of PTEN and VEGF proteins in hepatocellular carcinoma and its clinial significance

目的 研究与张力蛋白和辅助蛋白同源、第 10号染色体丢失的磷酸酶基因 (phosphatase and tensionhomology deleted on chrom osom e ten,PTEN)和血管内皮生长因子 (vascular endothelial growth factor,VEGF)的表达产物在肝细胞癌 (hepatocellular carcinoma,HCC)、癌旁肝组织及正常肝组织中的表达和意义。方法 免疫组化 S- P法研究 4 7例 HCC、 9例癌旁组织和 6例正常肝组织中 PTEN、VEGF蛋白的表达情况。结果 PTEN在HCC、癌旁肝组织及正常肝组织中的阳性表达率分别为 76 .6 % (36 / 4 7) ,10 0 .0 % (9/ 9)和 10 0 .0 % (6 / 6 ) ,差异有显著性 (χ2 =11.971,P=0 .0 0 2 5 )。HCC组织中 ,PTEN表达与 TNM分期 (γ=- 0 .6 315 ,P=0 .0 0 0 0 )和肝内血管癌栓(γ=- 0 .4 2 97,P=0 .0 0 2 6 )呈负相关。 VEGF在 HCC、癌旁肝组织及正常肝组织的阳性表达率分别为 70 .2 % (33/4 7) ,10 0 .0 % (9/ 9) ,16 .7% (1/ 6 ) ,差异有显著性 (χ2 =7.4 18,P=0 .0 2 4 5 )。HCC组织中 ,VEGF的表达与 TNM分期 (γ=0 .5 971,P=0 .0 0 0 0 )呈正相关。 PTEN、VEGF表达与肿瘤大小、癌组织分化程度及肝硬化背景均无关。VEGF表达与肝内血管癌栓无关。 Spearm an相关分析发现 PTEN与 VEGF表达呈负相关 (γ=- 0 .390 7。

Objective To investigate the significance of expression product of PTEN and VEGF proteins in hepatocellular carcinoma(HCC),paracancerous tissue and normal hepatic tissue.Methods PTEN and VEGF expressions were studied in 47 cases of HCC, 9 specimens of paracancerous tissue and 6 specimens of normal hepatic tissue using S-P immunohistochemistry.Results The positive rates of PTEN in HCC, paracancerous liver tissue and normal hepatic tissue were 76.6%(36/47),100.0%(9/9)and 100.0%(6/6),respectively(χ 2=11.971,P=0.0025). In HCC, the expression of PTEN was negatively correlated with TNM stage(γ=-0.6315, P=0.0000) and intrahepatic vascular embolism (γ=-0.4297,P=0.0026).The positive rates of VEGF in HCC, paracancerous liver tissue and normal hepatic tissue were 70.2% (33/47),100.0%(9/9) and 16.7%(1/6), respectively(χ 2=7.418,P=0.0245). In HCC the expression of VEGF was positively correlated with TNM stage (γ=0.5971, P=0.0000).No significant association was observed in expression of PTEN and VEGF with tumor size, the extent of differentiation in cancerous tissue and cirrhosis background. PTEN expression was not correlated with intrahepatic vascular embolism.Spearman correlation analysis revealed that PTEN was negatively correlated with VEGF expression(γ=-0.3907,P=0.0066).Conclusion The aberrant expression of PTEN and VEGF may play a role in occurrence, progression and intrahepatic metastasis of hepatocellular carcinoma.