微生物接种剂和酶制剂对黑麦草青贮发酵品质的影响

Effects of Microbial Inoculants and Enzyme Preparation Applied to Ryegrass at Ensiling on Silage Fermentation Quality

通过在室温23±2℃条件下添加乳杆菌、乳球菌和纤维素酶青贮黑麦草,以评价其单独或联合应用对黑麦草青贮发酵品质的影响.试验分为4组:对照组、乳杆菌组、乳杆菌和乳球菌复合添加组及乳杆菌和纤维素酶复合添加组.结果表明:单独添加乳杆菌、乳杆菌与乳球菌复合添加对黑麦草pH值无明显影响(P>0.05),但发酵质量较对照组有所改善.与对照组相比,乳杆菌和纤维素酶的复合添加明显降低了pH值(P<0.05),增加了发酵产物乳酸(P<0.01)的含量,明显降低了NH3-N(P<0.05)、NDF(P<0.01)和ADF(P<0.05)的含量,蛋白质损失减少约8%,但丁酸含量略高于其他组.因此,乳杆菌和纤维素酶联合添加可显著改善黑麦草青贮发酵品质.

Ryegrass was ensiled at 23±2 ℃ in greenhouse to evaluate the effects of lactobacillus, lactococcus and cellulose enzyme on fermentation quality. Ryegrass were treated with additional of single lactobacillus, lactobacillus plus lactococcus and lactobacillus plus cellulose enzyme, respectively, silage with no additives served as a control. The results showed that supplementation of single lactobacillus and lactobacillus plus lactococcus did not influence pH(P>0.05)of silage, but fermentation quality were improved slightly compared with that of control group. The pH of Lactobacillus plus cellulose enzyme group decreased (P<0.05) significantly during the whole silage fermentation, compared with that of control group. The lactic acid content was increased greatly(P<0.01) ,the contents of NH(3)-N(P<0.05),NDF (P<0.01) and ADF(P<0.05) were reduced significantly, protein loss was decreased about 8%,but the concentration of butyric acid was a little higher in lactobacillus plus cellulose enzyme group than those in other groups. It was concluded that additional of lactobacillus plus cellulose enzyme may improve the fermentation quality of ryegrass silage significantly.