健心口服液对心力衰竭家兔心肌iNOS mRNA表达的干预

Experimental Study on Intervetion Effect of Jianxin Oral Liquid on Gene Expression of iNOS of Myocardium in Rabbits with CHF

目的观察心力衰竭(CHF)家兔心肌诱生型一氧化氮合酶(iNOS)的基因表达与心功能的相关性及健心口服液的作用。方法选取家兔30只,随机分为正常空白组(空白组)、模型组、健心口服液组(试验组)三组,后两组造模后,试验组灌胃健心口服液浓缩液,模型组灌胃生理盐水;末次给药24h后,检测兔左心室功能,开胸取心室检测心室肌组织iNOSmRNA的表达。结果与空白组比较,模型组心室肌组织iNOSmRNA的表达量显著增加,心肌iNOSmRNA的表达量与每搏输出量(SV)、射血分数(EF)、心指数(CI)呈高度负相关性;与模型组比较,试验组心室肌组织iNOSmRNA表达量显著减少,心输出量(CO)、左室短轴缩短率(FS)、CI显著增加。结论CHF家兔模型心室肌组织iNOSmRNA的表达量明显增加,且与心功能呈高度负相关。健心口服液能够有效下调心肌组织iNOSmRNA的表达,减轻NO对心肌的负性肌力作用,从而改善心功能。

To observe the correlation between gene expression of iNOS in rabbits with CHF and cardiac function and the effect of Jianxin Oral Liquid(健心口服液). Methods:30 rabbits were randomly divided into 3 groups (the normal group,the model group and the Jianxin Oral Liquid group). After the heart failure models were established by injection of vein with ADM,the trial group was treated by Jianxin Oral Liquid,and the model normal saline. After 24 hours the two groups were treated for the last time,left ventricular functions and gene expression of iNOS in ventricular muscle tissues of all the groups were determined. Results:Compared with the normal group,the expression quantity of iNOSmaRNA in ventricular muscle tissues of the model group increased maredly,and there wss significant negative correlation between the expression quantity of iNOSmRNA and stroke volume (SV),ejection fraction (EF),cardiac index (CI);compared with the model group,the expression quantity of iNOSmRNA in ventricular muscle tissues of the trial group decreased markedly,and SV,CO,EF,FS and CI of the trial group increased markedly. Conclusion:The expression quantity of iNOSmRNA in ventricular muscle tissues of the CHF model rabbits increased markedly,and there was significant negative correlation between the expression quantity of iNOSmRNA and cardiac function.Jianxin Oral Liquid can lower the expression of iNOSmRNA and relieve the negative action of NO to myocardial so as go improve cardiac function.