轮状病毒检测技术

Advances in the Detection Techniques for Rotaviruses

轮状病毒是人和动物急性腹泻的重要病原 ,对轮状病毒进行快速、准确的检测对于疾病监测和疫情控制极为重要。从病原学、免疫学以及基因检测三方面 ,总结了轮状病毒检测技术的发展状况 ,重点介绍了较为成熟的免疫学技术 ,最新发展的实时荧光定量PCR、核酸序列依赖的扩增等分子生物学新技术 。

Rotaviruses are the major etiological agents of acute diarrhea illness of human and animals worldwide, which are associated with estimated 870,000 deaths every year among infants and young children under 3 years old. Apart from causing severe clinical disease, rotaviruses also lead to subclinical infections in man and animals. Further more, rotaviruses have been shown to be transmitted from the original host to a heterologous one. So it is necessary to detect rotaviruses rapidly and accurately from clinical and environmental samples. Due to the great impact on public health, it will be a long term task to control and prevent the diseases caused by the viruses. The advances in the field of detection of rotaviruses, including immunological and molecular biological techniques, especially the latest technology such as real time PCR, nucleic acid sequence based amplification (NASBA), etc were reviewed.;