摘要
载体骨架序列等非目的基因外源DNA片段整合入植物染色体中有可能引发的安全性问题已成为近年来植物基因工程研究的热点之一。总结了载体骨架序列整合进植物染色体的现象、由此引发的安全性隐患和机理 ,以及解决该问题的研究思路和方法 。
Biosafety issues induced by vector backbone sequences integration was one of the hotspots in recent transgenic plant research.The integration of vector backbone sequences in plant chromosome and the mechanism and biosafety related to it were discussed.Methods and strategies currently developed to solve this problem,especially minimal cassette transformation which could get rid of vector backbone sequences were further described.;
出处
《中国生物工程杂志》
CAS
CSCD
2004年第5期38-42,共5页
China Biotechnology
基金
辽宁省自然科学基金资助项目 ( 2 0 0 110 10 0 1)