转基因植物中载体骨架序列的安全性隐患及解决方案

Biosafety Issues and Management of Vector Backbone in Transgenic Plants

载体骨架序列等非目的基因外源DNA片段整合入植物染色体中有可能引发的安全性问题已成为近年来植物基因工程研究的热点之一。总结了载体骨架序列整合进植物染色体的现象、由此引发的安全性隐患和机理 ,以及解决该问题的研究思路和方法 。

Biosafety issues induced by vector backbone sequences integration was one of the hotspots in recent transgenic plant research.The integration of vector backbone sequences in plant chromosome and the mechanism and biosafety related to it were discussed.Methods and strategies currently developed to solve this problem,especially minimal cassette transformation which could get rid of vector backbone sequences were further described.;